Parameter	Standard	Method of Analysis
WSSV	Absent	Nested PCR/ real time PCR
AHPNFD/EHP	Absent	Nested PCR/ real time PCR
Stress test - Salinity	More than 90%	50% salinity drop for 1 hr
Stress test - Formalin	More than 90%	100 ppm formalin for 1 hr
Muscle to Gut ratio	4:1	Microscopy
Hepatopancreas	Full with oil globules	Microscopy
Gut	Full & without Gregarines	Microscopy
Necrosis	Absent	Microscopy
Fouling	Absent	Microscopy
Dorsal Rostral Spines	More than 4	Microscopy
Total length	More than 12 mm	Physical Observation
Size variation	Less than 5%	Physical Observation