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Product Technical Bulletin


DEGRADE–HY is a blend of hydrocarbon degrading bacteria comprising of both gram positive and gram negative species. They are primarily used in soil remediation of mixtures of crude oil, tar, alcohols, ketones and other cyclic products of petrochemical origin.
As of petroleum products remediation is concerned the character of the treatment sites vary dramatically so a “bench test” is always advised before going for a large scale application.
Bacteria Count 3 Billion Colony Forming Units/gram
Appearance Free flowing brown powder
Odor Yeast Like
  • Rapidly degrades all major components of gasoline, diesel, #4, #5, & #6 fuel oils. Also degrades phthalates, ketones, various solvents, sulfur-containing compounds, alcohols, chlorinated hydrocarbons, amines, and other complex organic compounds found in refineries and chemical plant wastewater, which are toxic to naturally occurring bacteria.
  • Reduces further environmental damage by degrading the contaminant plume.
    Cultures can tolerate petroleum concentrations inhibitory to many indigenous populations.
  • Can tolerate up to 20 ppm concentration of heavy metals, without the need for bench testing. To verify tolerance of heavy metals from 20 ppm to 1,000 ppm, perform Alken-Murray's proprietary soil bench test procedure.
  • Effective over a wide temperature range (40 to 100 F; optimum 75± 10 F)
  • Effective over a wide pH range (6.0 to 8.5; optimum 7.1 ± 0.3)
Non-toxic, non-corrosive, safe to handle and store when used as directed.

For bioremediation of petroleum compounds, maintain at least 1ppm of ammonia nitrogen and 0.5 ppm of Orthophosphate to give a good start up for the bioremediation of the site.

The strains in this formula degrade hydrocarbons aerobically, requiring at least 1 ppm of oxygen, with 2 to 5 ppm being ideal. Hydrogen sulfide remediation can be performed both aerobically and anaerobically by this formula.

For optimum bacterial performance, hydrate DEGRADE– HY before application, thoroughly mix each kilogram of dry product with 10 liters of warm (approx.30oC) water without chlorine. Allow the mixture to hydrate for two hours, stirring vigorously every 30 minutes, or else stir continuously for 2 hours. For soil applications, use of a slow-release calcium or magnesium peroxide product will deliver optimum results. Call SALEM MICROBES PVT LTD for your requirement on this product.

For application to land spills : SALEM MICROBES PVT LTD suggests that you obtain the services of an environmental engineering firm for geological and engineering assessment and application, to ensure safety and compliance with all regulations. Dosage per cubic meter of soil contaminations is 100- 150 grams, applied once a month, until soil remediate.

If you have surface spills on paved or Concrete carpeted area, call SALEM MICROBES PVT LTD, we will be in a position to offer a surfactant blended product for this application, in this blend microbes capable of degrading long-chain fatty acids and petroleum-hydrocarbon degrading Bacillus are also included.

For general application rates for lagoons, activated sludge systems, lakes or ponds ; which have been contaminated by a petroleum oil spill. Mechanical aeration is required for treatment of petroleum hydrocarbon contaminated wastewater, or other aquatic environments. Since this product contains no surfactants or pathogenic bacteria, it is safe to apply DEGRADE–HY to lakes, ponds or the ocean, where fish and wildlife are present.
Avoid use of algaecides, herbicides, and antibiotics when using any DEGRADE RANGE of products.

DEGRADE–HY is packed in 25 Kgs and 50 Kgs drums. Store in a cool dry place. Avoid inhalation of dust. Wash hands with warm, soapy water after handling.